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Title

Expressed sequence tags from heat-shocked seagrass Zostera noltii (Hornemann) from its southern distribution range

Publication Year

2011

Author(s)
  • Massa, Sonia I.
  • Pearson, Gareth A.
  • Aires, Tania
  • Kube, Michael
  • Olsen, Jeanine L.
  • Reinhardt, Richard
  • Serrao, Ester A.
  • Arnaud-Haond, Sophie
Source
MARINE GENOMICS Volume: 4 Issue: 3 Pages: 181-188 Published: 2011
ISSN
1874-7787
Abstract

Predicted global climate change threatens the distributional ranges of species worldwide. We identified genes expressed in the intertidal seagrass Zostera midi during recovery from a simulated low tide heat-shock exposure. Five Expressed Sequence Tag (EST) libraries were compared, corresponding to four recovery times following sub-lethal temperature stress, and a non-stressed control. We sequenced and analyzed 7009 sequence reads from 30 min, 2 h, 4 h and 24 h after the beginning of the heat-shock (AHS), and 1585 from the control library, for a total of 8594 sequence reads. Among 51 Tentative UniGenes (TUGs) exhibiting significantly different expression between libraries, 19 (37.3%) were identified as 'molecular chaperones' and were over-expressed following heat-shock, while 12 (23.5%) were 'photosynthesis TUGs' generally under-expressed in heat-shocked plants. A time course analysis of expression showed a rapid increase in expression of the molecular chaperone class, most of which were heat-shock proteins; which increased from 2 sequence reads in the control library to almost 230 in the 30 min AHS library, followed by a slow decrease during further recovery. In contrast, 'photosynthesis TUGs' were under-expressed 30 min AHS compared with the control library, and declined progressively with recovery time in the stress libraries, with a total of 29 sequence reads 24 h AHS, compared with 125 in the control. A total of 4734 TUGs were screened for EST-Single Sequence Repeats (EST-SSRs) and 86 microsatellites were identified. (C) 2011 Elsevier B.V. All rights reserved.

Author Keyword(s)
  • EST library
  • High temperature stress
  • Abiotic stress response
  • Climate change
KeyWord(s) Plus
  • ESCHERICHIA-COLI
  • PHOTOSYNTHETIC RESPONSE
  • MOLECULAR CHAPERONES
  • TEMPERATURE STRESS
  • HALOPHILA-OVALIS
  • GENE-EXPRESSION
  • PROTEINS
  • ECOSYSTEMS
  • EVOLUTION
  • SURVIVAL
ESI Discipline(s)
  • Molecular Biology & Genetics
  • Plant & Animal Science
Web of Science Category(ies)
  • Genetics & Heredity
  • Marine & Freshwater Biology
Adress(es)

[Arnaud-Haond, Sophie] IFREMER, Ctr Brest, F-29280 Plouzane, France; [Olsen, Jeanine L.] Univ Groningen, Dept Marine Benth Ecol & Evolut, Ctr Ecol & Evolutionary Studies, Ctr Biol, NL-9750 AA Haren, Netherlands; [Kube, Michael; Reinhardt, Richard] Max Planck Inst Mol Genet, D-14195 Berlin, Germany; [Massa, Sonia I.; Pearson, Gareth A.; Aires, Tania; Serrao, Ester A.] Univ Algarve, CCMAR CIMAR, P-8005139 Faro, Portugal

Reprint Adress

Arnaud-Haond, S (reprint author), IFREMER, Ctr Brest, BP 70, F-29280 Plouzane, France.

Country(ies)
  • France
  • Germany
  • Netherlands
  • Portugal
CNRS - Adress(es)

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Accession Number
WOS:000294985500007
uid:/31Z3KTFV
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